The Human EPOR (Erythropoietin Receptor) CLIA Kit is specifically designed for the accurate detection of Erythropoietin Receptor levels in human serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a wide variety of research applications.The Erythropoietin Receptor is a critical component involved in the regulation of erythropoiesis, or red blood cell production.
It plays a key role in conditions such as anemia, kidney disease, and cancer, making it a vital biomarker for studying these diseases and potential therapeutic interventions.With the Human EPOR CLIA Kit, researchers can confidently measure Erythropoietin Receptor levels with precision and accuracy, advancing their understanding of biological processes and disease mechanisms. Benefit from reliable results and robust performance with this cutting-edge assay kit.
Product Name:
Human EPOR (Erythropoietin Receptor) CLIA Kit
SKU:
HUES00799
Target:
Human EPOR (Erythropoietin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPOR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPOR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPOR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPOR. You can calculate the concentration of Human EPOR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-104
87-102
95-106
Average (%)
95
94
101
1:4
Range (%)
96-109
88-101
91-107
Average (%)
102
96
97
1:8
Range (%)
91-103
89-103
103-119
Average (%)
96
96
109
1:16
Range (%)
89-103
95-111
92-102
Average (%)
96
103
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.0
196.98
806.38
107.93
180.72
763.06
Standard deviation
11.32
22.67
64.11
12.76
18.4
77.76
C V (%)
10.78
11.51
7.95
11.82
10.18
10.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPOR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human EPOR in samples. No significant cross-reactivity or interference between Human EPOR and analogues was observed.
