The Human EPYC (Epiphycan) CLIA Kit is a cutting-edge assay designed for the precise detection of EPYC levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, making it the perfect choice for a variety of research applications.EPYC, also known as Epiphycan, is a key protein involved in various biological processes, including cartilage development and maintenance.
Its dysregulation has been linked to conditions such as osteoarthritis and skeletal dysplasias, highlighting its importance as a potential biomarker for studying these diseases and developing targeted treatments.Overall, the Human EPYC (Epiphycan) CLIA Kit offers researchers a reliable tool for investigating the role of EPYC in health and disease, paving the way for advancements in the field of musculoskeletal research.
Product Name:
Human EPYC (Epiphycan) CLIA Kit
SKU:
HUES00265
Target:
Human EPYC (Epiphycan)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPYC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPYC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPYC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human EPYC. You can calculate the concentration of Human EPYC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
95-109
84-95
Average (%)
91
100
90
1:4
Range (%)
96-110
90-103
86-101
Average (%)
102
97
92
1:8
Range (%)
98-114
103-120
86-100
Average (%)
104
110
93
1:16
Range (%)
96-110
85-97
89-104
Average (%)
104
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-101
92
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
84-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.35
296.53
741.01
104.43
289.51
712.34
Standard deviation
9.12
34.25
56.91
9.05
32.11
58.84
C V (%)
8.34
11.55
7.68
8.67
11.09
8.26
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human EPYC concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human EPYC in samples. No significant cross-reactivity or interference between Human EPYC and analogues was observed.
