The Human ES (Endostatin) CLIA Kit is specifically designed for the precise measurement of endostatin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reliable results, making it a valuable tool for various research applications.Endostatin is a key player in angiogenesis regulation, inhibiting blood vessel formation and impacting cell growth.
Its implications in cancer, cardiovascular diseases, and other health conditions highlight its importance as a biomarker for studying and developing treatments for these diseases.This Human ES CLIA Kit from Assay Genie provides researchers with a powerful tool for exploring the role of endostatin in health and disease, offering a comprehensive solution for measuring this crucial protein in biological samples.
Product Name:
Human ES (Endostatin) CLIA Kit
SKU:
HUES00061
Target:
Human ES (Endostatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ES. You can calculate the concentration of Human ES in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
95-111
89-104
Average (%)
98
101
95
1:4
Range (%)
94-108
96-110
90-105
Average (%)
101
104
97
1:8
Range (%)
86-99
100-117
100-118
Average (%)
92
107
107
1:16
Range (%)
100-115
97-109
98-111
Average (%)
108
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
90-107
98
Cell culture media (n=5)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.72
339.47
1770.97
193.38
345.78
1802.39
Standard deviation
23.49
29.7
142.03
16.42
26.52
140.41
C V (%)
12.65
8.75
8.02
8.49
7.67
7.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ES concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ES in samples. No significant cross-reactivity or interference between Human ES and analogues was observed.
