Human ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit
The Human ESM1 (Endothelial Cell-Specific Molecule-1) CLIA Kit is specifically designed for the precise measurement of ESM1 levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.ESM1 is a key protein that is predominantly expressed in endothelial cells and plays a critical role in the regulation of vascular endothelial function. It is involved in processes such as angiogenesis, inflammation, and vascular permeability, making it a valuable biomarker for studying conditions like cancer, cardiovascular diseases, and inflammatory disorders.
By utilizing the Human ESM1 CLIA Kit, researchers can gain valuable insights into the role of ESM1 in various physiological and pathological processes, ultimately leading to a better understanding of disease mechanisms and the development of potential therapeutic strategies.
Product Name:
Human ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit
SKU:
HUES00884
Target:
Human ESM1 (Endothelial Cell Specific Molecule 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
87-98
100-115
Average (%)
102
92
108
1:4
Range (%)
84-97
93-109
95-110
Average (%)
91
100
101
1:8
Range (%)
100-117
98-110
92-107
Average (%)
107
103
98
1:16
Range (%)
88-100
83-99
91-106
Average (%)
93
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.54
45.15
245.07
22.75
43.28
231.41
Standard deviation
3.02
3.9
19.88
2.81
3.14
25.41
C V (%)
12.83
8.64
8.11
12.35
7.26
10.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
