Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit
The Human ESM1 (Endothelial Cell-Specific Molecule-1) ELISA Kit is specifically designed for the precise measurement of ESM1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.ESM1 is a key protein that is primarily expressed in endothelial cells and is involved in various physiological processes, including angiogenesis and inflammation. Dysregulation of ESM1 has been linked to diseases such as cancer, inflammatory disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With the Human ESM1 ELISA Kit, researchers can accurately quantify ESM1 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. This kit is a valuable tool for scientists and clinicians alike looking to advance their understanding of ESM1 biology and its implications in human health and disease.
Product Name:
Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit
SKU:
HUES02571
Target:
Human ESM1 (Endothelial Cell Specific Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ESM1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ESM1. You can calculate the concentration of Human ESM1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
91-105
96-110
Average (%)
95
97
103
1:4
Range (%)
91-105
79-93
83-94
Average (%)
97
86
88
1:8
Range (%)
92-108
87-98
84-95
Average (%)
99
93
89
1:16
Range (%)
90-101
88-101
88-102
Average (%)
95
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
100
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
92-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.84
154.68
423.76
52.25
168.48
438.78
Standard deviation
3.49
8.8
13.01
3.18
10.08
22.2
C V (%)
6.36
5.69
3.07
6.09
5.98
5.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ESM1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ESM1 in samples. No significant cross-reactivity or interference between Human ESM1 and analogues was observed.
