The Human ET-1 (Endothelin-1) CLIA Kit is a cutting-edge tool designed for the precise measurement of endothelin-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Endothelin-1 is a key peptide known for its role in vasoconstriction and the regulation of blood pressure. Dysregulation of endothelin-1 levels has been linked to various cardiovascular diseases, including hypertension and heart failure, highlighting its importance as a biomarker in cardiovascular research.
By utilizing the Human ET-1 CLIA Kit, researchers can gain valuable insights into the pathophysiology of cardiovascular disorders and explore potential therapeutic interventions targeting the endothelin-1 pathway. With its reliability and performance, this kit is an essential tool for advancing our understanding of endothelin-1 biology and its implications in human health.
Product Name:
Human ET-1 (Endothelin 1) CLIA Kit
SKU:
HUES00062
Target:
Human ET-1 (Endothelin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
101-113
87-101
Average (%)
96
107
93
1:4
Range (%)
85-98
100-114
92-107
Average (%)
92
106
99
1:8
Range (%)
93-106
88-102
99-114
Average (%)
100
94
104
1:16
Range (%)
85-97
96-110
96-112
Average (%)
90
104
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
101-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.86
62.35
206.66
23.83
67.8
198.69
Standard deviation
2.46
6.53
20.48
2.29
7.92
16.23
C V (%)
10.76
10.47
9.91
9.61
11.68
8.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ET-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
