The Human F-Box Protein 32 (FBXO32) ELISA Kit is specifically designed to measure the levels of FBXO32 in human serum, plasma, and cell culture supernatants with high accuracy. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.FBXO32 is a key player in cellular processes, regulating the degradation of specific proteins and influencing cell signaling pathways. Dysregulation of FBXO32 has been linked to various diseases, including cancer, cardiac disorders, and muscle wasting conditions, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its reliable performance and easy-to-use format, the Human FBXO32 ELISA Kit is an indispensable tool for researchers investigating the role of FBXO32 in health and disease. Get precise and insightful data with this innovative ELISA kit from Assay Genie.
Product Name:
Human F-Box Protein 32 (FBXO32) ELISA Kit
Product Code:
HUDL01033
Size:
96 Assays
Target:
FBXO32
Synonyms:
ATROGIN1, Fbx32, MAFbx, Muscle atrophy F-box protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
