Human F13A1 (Coagulation Factor 13 A1 Polypeptide) CLIA Kit
The Human F13A1 (Coagulation Factor 13 A1) CLIA Kit is specifically designed for the precise quantitative measurement of F13A1 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research purposes.Coagulation Factor 13 A1 is an essential component in the blood clotting process, playing a critical role in the formation of stable fibrin clots. Dysregulation of F13A1 levels has been implicated in various coagulation disorders and thrombotic diseases, making it a valuable biomarker for diagnostic and research purposes.
Whether investigating coagulation disorders, studying thrombosis-related diseases, or exploring potential therapeutic interventions, the Human F13A1 CLIA Kit provides researchers with a reliable and efficient solution for measuring F13A1 levels in human samples. Invest in this kit today to enhance your research capabilities and gain valuable insights into the role of F13A1 in health and disease.
Product Name:
Human F13A1 (Coagulation Factor 13 A1 Polypeptide) CLIA Kit
SKU:
HUES00481
Target:
Human F13A1 (Coagulation Factor 13 A1 Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human F13A1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human F13A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human F13A1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human F13A1. You can calculate the concentration of Human F13A1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
96-110
98-113
Average (%)
104
101
103
1:4
Range (%)
98-115
100-115
85-99
Average (%)
106
106
91
1:8
Range (%)
102-119
100-116
102-118
Average (%)
109
106
108
1:16
Range (%)
85-95
100-116
99-112
Average (%)
90
109
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.66
72.96
305.26
40.7
73.29
277.75
Standard deviation
4.56
7.3
34.4
3.51
6.63
23.64
C V (%)
12.11
10.01
11.27
8.62
9.05
8.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human F13A1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed.
