Human FABP5 (Fatty Acid Binding Protein 5, Epidermal) CLIA Kit
The Human FABP5 (Fatty Acid Binding Protein 5) CLIA Kit is designed for the accurate detection of FABP5 levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FABP5 is a key protein involved in fatty acid transport and metabolism, playing a vital role in lipid signaling pathways and cellular functions. Dysregulation of FABP5 has been linked to various diseases, including cancer, metabolic disorders, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and identifying potential therapeutic targets.
By utilizing the Human FABP5 CLIA Kit, researchers can gain valuable insights into the role of FABP5 in health and disease, paving the way for innovative research and drug development strategies. Trust in the reliability and accuracy of this kit to advance your scientific investigations and uncover new possibilities in the field of molecular biology.
Product Name:
Human FABP5 (Fatty Acid Binding Protein 5, Epidermal) CLIA Kit
SKU:
HUES00638
Target:
Human FABP5 (Fatty Acid Binding Protein 5, Epidermal )
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FABP5. You can calculate the concentration of Human FABP5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
88-103
86-99
Average (%)
101
95
92
1:4
Range (%)
86-100
100-113
100-118
Average (%)
92
106
108
1:8
Range (%)
93-106
85-97
86-100
Average (%)
99
92
93
1:16
Range (%)
89-101
98-114
98-112
Average (%)
94
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
106
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.65
640.9
1925.48
216.47
642.07
2075.49
Standard deviation
25.77
73.0
203.72
27.15
73.07
130.34
C V (%)
12.53
11.39
10.58
12.54
11.38
6.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FABP5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FABP5 in samples. No significant cross-reactivity or interference between Human FABP5 and analogues was observed.
