Human Family With Sequence Similarity 3,Member B (FAM3B) ELISA Kit
The Human Family with Sequence Similarity 3, Member B (FAM3B) ELISA Kit is a powerful tool for the precise measurement of FAM3B levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an ideal choice for a wide array of research studies.FAM3B is a member of the FAM3 family of proteins and is known to play a crucial role in various biological processes such as cell proliferation, differentiation, and metabolism.
Dysregulation of FAM3B has been associated with several diseases including diabetes, cancer, and obesity, making it a valuable biomarker for understanding these conditions and identifying potential therapeutic targets.The Human Family with Sequence Similarity 3, Member B (FAM3B) ELISA Kit is a valuable tool for researchers looking to explore the role of FAM3B in health and disease, with its reliable performance and accurate results ensuring confidence in experimental outcomes.
Product Name:
Human Family With Sequence Similarity 3,Member B (FAM3B) ELISA Kit
Product Code:
HUDL01017
Size:
96 Assays
Target:
FAM3B
Synonyms:
ORF9, PANDER, PRED44, C21orf11, C21orf76, Cytokine-like protein 2-21, Pancreatic-derived factor
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
