Human FAS/CD95 (Factor Related Apoptosis) CLIA Kit
The Human FAS (CD95) Factor-Related Apoptosis CLIA Kit is a cutting-edge assay designed for the accurate measurement of FAS (CD95) levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.FAS (CD95) is a critical protein involved in apoptosis, the process of programmed cell death. Dysregulation of FAS signaling has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
Therefore, measuring FAS levels can provide valuable insights into disease mechanisms and potential therapeutic interventions.With its user-friendly format and superior performance, the Human FAS (CD95) Factor-Related Apoptosis CLIA Kit is an indispensable tool for researchers and clinicians studying apoptosis-related pathways and developing novel treatments. Order yours today and unlock the potential of FAS (CD95) in your research endeavors.
Product Name:
Human FAS/CD95 (Factor Related Apoptosis) CLIA Kit
SKU:
HUES00065
Target:
Human FAS/CD95 (Factor Related Apoptosis)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FAS/CD95. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FAS/CD95, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FAS/CD95. You can calculate the concentration of Human FAS/CD95 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
92-107
96-109
Average (%)
102
99
103
1:4
Range (%)
97-113
95-109
97-108
Average (%)
103
101
103
1:8
Range (%)
85-99
93-110
92-108
Average (%)
92
101
98
1:16
Range (%)
102-116
102-120
91-105
Average (%)
109
109
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
98-109
104
Cell culture media (n=5)
98-110
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.71
144.43
466.8
52.94
149.22
443.94
Standard deviation
4.31
14.76
49.11
4.46
10.71
27.35
C V (%)
8.67
10.22
10.52
8.42
7.18
6.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FAS/CD95 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FAS/CD95 in samples. No significant cross-reactivity or interference between Human FAS/CD95 and analogues was observed.
