Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) CLIA Kit
The Human FASL (TNFSF6) Factor-Related Apoptosis Ligand CLIA Kit is designed for the precise measurement of FASL levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research applications.FASL, also known as TNFSF6, is a vital protein involved in inducing apoptosis (programmed cell death) in certain cells. It plays a crucial role in immune responses, specifically in the regulation of cell death in lymphocytes.
Dysregulation of FASL expression has been linked to various diseases, including autoimmune disorders and cancer, making it a significant biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human FASL Factor-Related Apoptosis Ligand CLIA Kit is a valuable tool for researchers looking to investigate the role of FASL in disease pathogenesis and develop targeted treatment strategies.
Product Name:
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) CLIA Kit
SKU:
HUES00066
Target:
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FASL/TNFSF6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FASL/TNFSF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FASL/TNFSF6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FASL/TNFSF6. You can calculate the concentration of Human FASL/TNFSF6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
85-97
84-96
Average (%)
94
91
91
1:4
Range (%)
96-111
93-107
95-109
Average (%)
101
100
102
1:8
Range (%)
91-103
103-118
91-104
Average (%)
97
110
98
1:16
Range (%)
99-111
88-102
92-106
Average (%)
105
94
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.86
87.22
336.36
43.51
94.58
337.08
Standard deviation
4.4
9.11
37.94
4.45
10.03
30.2
C V (%)
10.03
10.44
11.28
10.23
10.6
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FASL/TNFSF6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FASL/TNFSF6 in samples. No significant cross-reactivity or interference between Human FASL/TNFSF6 and analogues was observed.
