Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit
The Human FASL (TNFSF6) ELISA Kit is specifically designed for the precise measurement of FASL levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit guarantees accurate and consistent results, making it well-suited for a variety of research purposes.FASL, also known as FAS ligand, plays a critical role in the regulation of apoptosis, or programmed cell death. It is a key player in the immune system, aiding in the elimination of unwanted or damaged cells.
Dysregulation of FASL has been linked to various diseases, including autoimmune disorders and cancer, underscoring its importance as a biomarker in studying these conditions and potential therapeutic interventions.With its reliable performance and versatility, the Human FASL (TNFSF6) ELISA Kit offers researchers a valuable tool for investigating the role of FASL in health and disease, ultimately contributing to advancements in medical research and treatment strategies.
Product Name:
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand) ELISA Kit
SKU:
HUES01355
Target:
Human FASL/TNFSF6 (Factor Related Apoptosis Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FASL/TNFSF6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FASL/TNFSF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FASL/TNFSF6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FASL/TNFSF6. You can calculate the concentration of Human FASL/TNFSF6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
93-106
90-103
Average (%)
103
99
96
1:4
Range (%)
90-106
81-91
85-97
Average (%)
98
86
92
1:8
Range (%)
88-99
88-100
81-93
Average (%)
94
93
87
1:16
Range (%)
89-103
82-95
82-96
Average (%)
96
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
102
EDTA plasma (n=5)
93-109
100
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.15
142.3
454.75
50.49
135.64
412.66
Standard deviation
2.94
8.24
14.92
2.9
6.47
15.76
C V (%)
5.86
5.79
3.28
5.74
4.77
3.82
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FASL/TNFSF6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FASL/TNFSF6 in samples. No significant cross-reactivity or interference between Human FASL/TNFSF6 and analogues was observed.
