Human Fatty Acid Binding Protein 8,Myelin (FABP8) ELISA Kit
The Human FABP8 ELISA Kit is specifically designed for the precise detection of Human Fatty Acid Binding Protein 8 (FABP8) levels in biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.FABP8, also known as Myelin FABP, is a vital protein involved in the transportation and metabolism of fatty acids within cells, particularly in the nervous system.
Dysregulation of FABP8 has been associated with neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.By utilizing the Human FABP8 ELISA Kit, researchers can effectively measure FABP8 levels in biological samples, providing valuable insights into its role in health and disease. This kit is essential for advancing research in neurology, lipid metabolism, and related fields.
Product Name:
Human Fatty Acid Binding Protein 8,Myelin (FABP8) ELISA Kit
Product Code:
HUDL01009
Size:
96 Assays
Target:
FABP8
Synonyms:
PMP2, M-FABP, MFABP, MP2, P2, Peripheral Myelin Protein 2, Myelin P2 Protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
