Human Fatty Acid Transport Protein 4 (FATP4) ELISA Kit
The Human Fatty Acid Transport Protein 4 (FATP4) ELISA Kit is a highly reliable and sensitive tool for the quantitative detection of FATP4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring precise and reproducible results for various research applications.FATP4 is an essential protein involved in the transport of fatty acids into cells, playing a key role in lipid metabolism and energy production. Dysregulation of FATP4 has been linked to various metabolic disorders, including obesity, diabetes, and cardiovascular diseases, making it a valuable marker for studying these conditions and developing potential therapeutic interventions.
The Human FATP4 ELISA Kit is a valuable tool for researchers looking to investigate the role of FATP4 in metabolic disorders and explore potential treatment strategies. With its high sensitivity and specificity, this kit provides reliable data for advancing our understanding of FATP4 function and its implications in human health and disease.
Product Name:
Human Fatty Acid Transport Protein 4 (FATP4) ELISA Kit
Product Code:
HUDL01024
Size:
96 Assays
Target:
FATP4
Synonyms:
SLC27A4, FATP4, Long-Chain Fatty Acid Transport Protein 4, Solute Carrier Family 27 Member 4,Fatty Acid Transporter
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
