The Human FBLN1 (Fibulin 1) ELISA Kit is specifically designed for the accurate measurement of fibulin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research purposes.Fibulin-1 is a vital extracellular matrix protein that plays a crucial role in cell adhesion, migration, and signaling. It is involved in various biological processes, including tissue development, wound healing, and tumorigenesis.
Abnormal levels of fibulin-1 have been associated with various diseases, making it a valuable biomarker for studying these conditions and potentially developing new treatments.Overall, the Human FBLN1 (Fibulin 1) ELISA Kit is a reliable tool for researchers in the field of molecular biology and biomarker discovery, providing accurate quantification of fibulin-1 levels in biological samples.
Product Name:
Human FBLN1 (Fibulin 1) ELISA Kit
SKU:
HUES02674
Target:
Human FBLN1 (Fibulin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FBLN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FBLN1. You can calculate the concentration of Human FBLN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-111
87-102
Average (%)
95
105
94
1:4
Range (%)
92-104
86-100
84-95
Average (%)
98
91
89
1:8
Range (%)
86-98
82-96
83-96
Average (%)
93
88
89
1:16
Range (%)
91-105
83-95
83-94
Average (%)
99
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
94-110
102
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.98
4.26
16.72
2.03
4.62
15.87
Standard deviation
0.12
0.23
0.69
0.14
0.26
0.78
C V (%)
6.06
5.4
4.13
6.9
5.63
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FBLN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBLN1 in samples. No significant cross-reactivity or interference between Human FBLN1 and analogues was observed.
