The Human FBLN5 (Fibulin-5) CLIA Kit is a specialized assay designed for the accurate quantification of fibulin-5 levels in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for their studies.Fibulin-5 is a critical protein that plays a key role in maintaining the structural integrity of elastic fibers in various tissues, including blood vessels and skin.
Dysregulation of fibulin-5 expression has been linked to conditions such as cardiovascular diseases and skin disorders, making it a valuable biomarker for understanding these diseases and developing potential treatments.With the Human FBLN5 (Fibulin-5) CLIA Kit, researchers can accurately measure fibulin-5 levels in human samples, allowing for the investigation of its role in disease pathology and the development of targeted therapies.
Product Name:
Human FBLN5 (Fibulin 5) CLIA Kit
SKU:
HUES00721
Target:
Human FBLN5 (Fibulin 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FBLN5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBLN5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FBLN5. You can calculate the concentration of Human FBLN5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
84-98
86-101
Average (%)
95
90
92
1:4
Range (%)
100-114
86-102
90-105
Average (%)
106
93
96
1:8
Range (%)
87-100
103-120
98-110
Average (%)
93
110
104
1:16
Range (%)
98-113
87-100
104-119
Average (%)
104
92
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
98
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.01
4.64
16.36
2.03
5.09
17.42
Standard deviation
0.19
0.41
1.66
0.22
0.52
1.56
C V (%)
9.45
8.84
10.15
10.84
10.22
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FBLN5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FBLN5 in samples. No significant cross-reactivity or interference between Human FBLN5 and analogues was observed.
