Human Fc alphaR/CD89 (Receptor I for the Fc Fragment of IgA) CLIA Kit
The Human FC-alphaR/CD89 Receptor I for the Fc Fragment of IgA CLIA Kit (HUes00968) is a cutting-edge diagnostic tool designed for the accurate detection of the FC-alphaR/CD89 receptor I levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.The FC-alphaR/CD89 receptor I is a crucial component of the immune system, specifically involved in the binding and clearance of IgA antibodies.
Dysregulation of this receptor has been linked to autoimmune diseases, inflammatory conditions, and other immune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human FC-alphaR/CD89 Receptor I for the Fc Fragment of IgA CLIA Kit (HUes00968) is an essential tool for researchers and clinicians looking to gain insights into immune function and develop novel treatments for a range of diseases.
Product Name:
Human Fc alphaR/CD89 (Receptor I for the Fc Fragment of IgA) CLIA Kit
SKU:
HUES00968
Target:
Human Fc alphaR/CD89 (Receptor I for the Fc Fragment of IgA)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FcαR/CD89. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcαR/CD89 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcαR/CD89, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FcαR/CD89. You can calculate the concentration of Human FcαR/CD89 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
93-108
90-102
Average (%)
96
101
97
1:4
Range (%)
89-102
90-103
84-98
Average (%)
96
96
91
1:8
Range (%)
99-115
91-106
89-101
Average (%)
106
98
94
1:16
Range (%)
87-100
99-114
87-97
Average (%)
92
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
106
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
0.86
4.21
0.46
0.81
3.85
Standard deviation
0.05
0.09
0.47
0.05
0.07
0.41
C V (%)
10.87
10.47
11.16
10.87
8.64
10.65
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FcαR/CD89 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FcαR/CD89 in samples. No significant cross-reactivity or interference between Human FcαR/CD89 and analogues was observed.
