Human Fc Fragment Of IgA Receptor (FcaR) ELISA Kit
The Human FC Fragment of IgA Receptor (FCAR) ELISA Kit is a specialized assay designed for the accurate quantification of FCAR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.FCAR is a critical component involved in the recognition and binding of IgA antibodies, playing a crucial role in the immune response and inflammatory processes. Dysregulation of FCAR expression has been implicated in various inflammatory and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and ease of use, the Human FC Fragment of IgA Receptor (FCAR) ELISA Kit is an essential tool for researchers and clinicians looking to explore the role of FCAR in immune responses and inflammatory diseases. Get your kit today and unlock new possibilities in your research endeavors.
Product Name:
Human Fc Fragment Of IgA Receptor (FcaR) ELISA Kit
Product Code:
HUDL01034
Size:
96 Assays
Target:
FcaR
Synonyms:
CD89 FcaRI Receptor I For The Fc Region Of Immunoglobulin A IgA Fc receptor Immunoglobulin alpha Fc receptor
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
