Human Fc Fragment Of IgG Binding Protein (FcgBP) ELISA Kit
The Human Fc Fragment of IgG Binding Protein (FcGBP) ELISA Kit is specifically designed for the precise and sensitive measurement of FcGBP levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unparalleled accuracy and reproducibility, making it ideal for a wide range of research applications.FcGBP is a key protein that plays a vital role in the binding of IgG antibodies, thus influencing immune responses and inflammation. Dysregulation of FcGBP has been implicated in various diseases, including autoimmune disorders and infectious diseases.
Therefore, accurate measurement of FcGBP levels is crucial for studying these conditions and developing potential therapeutic interventions.With its high sensitivity and specificity, the Human FcGBP ELISA Kit provides researchers with a valuable tool for investigating the role of FcGBP in health and disease. Trust in this kit to deliver reliable and consistent results for your research needs.
Product Name:
Human Fc Fragment Of IgG Binding Protein (FcgBP) ELISA Kit
Product Code:
HUDL01036
Size:
96 Assays
Target:
FcgBP
Synonyms:
FC(GAMMA)BP, IgG Fc Binding Protein, Human Fc Gamma BP, Fcgamma-binding protein antigen
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
