Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit
The Human FC Fragment of IgG Low Affinity IIIB Receptor (FCGR3B) ELISA Kit is a reliable and sensitive assay designed for the accurate measurement of FCGR3B levels in human serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reproducible results for a variety of research applications.FCGR3B, also known as CD16b, is a key receptor involved in immune responses and plays a crucial role in antibody-dependent cellular cytotoxicity. Dysregulation of FCGR3B has been implicated in autoimmune diseases, infectious diseases, and inflammatory disorders, making it a valuable biomarker for investigating these conditions and developing potential therapeutic interventions.
With its high performance and ease of use, the Human FC Fragment of IgG Low Affinity IIIB Receptor (FCGR3B) ELISA Kit is an essential tool for researchers studying the function and regulation of FCGR3B in various disease states. Get accurate and reliable results with this advanced ELISA kit from AssayGenie.
Product Name:
Human Fc Fragment Of IgG Low Affinity IIIb Receptor (FcgR3B) ELISA Kit
Product Code:
HUDL01038
Size:
96 Assays
Target:
FcgR3B
Synonyms:
CD16b, CD16-B, FCGR3-B, FCG3, FcgRIII, Low affinity immunoglobulin gamma Fc region receptor III-B
Detection Method:
Sandwich
Range:
1.25-80ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
