The Human Fcγ (Fc Fragment of IgG) CLIA Kit is specifically designed for the accurate quantification of Fcγ levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.Fcγ, a key component in immune responses, plays a crucial role in antibody-mediated immune functions such as phagocytosis, antibody-dependent cellular cytotoxicity, and immune complex clearance. Dysregulation of Fcγ has been implicated in various autoimmune diseases, inflammatory disorders, and immunodeficiencies, making it a valuable biomarker for research and clinical applications.
With its user-friendly protocol and efficient performance, the Human Fcγ CLIA Kit is an essential tool for studying the role of Fcγ in immune responses, disease progression, and therapeutic interventions. Obtain accurate and reliable results with this advanced assay kit from Assay Genie. Visit the website to learn more: www.assaygenie.com/human-fcgamma-fc-fragment-of-igg-clia-kit-hues00971/
Product Name:
Human Fcgamma (Fc Fragment of IgG) CLIA Kit
SKU:
HUES00971
Target:
Human Fcgamma (Fc Fragment of IgG)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Fcγ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Fcγ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Fcγ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Fcγ. You can calculate the concentration of Human Fcγ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
102-114
87-100
Average (%)
104
108
94
1:4
Range (%)
104-117
98-114
98-115
Average (%)
110
106
106
1:8
Range (%)
100-115
90-107
89-102
Average (%)
105
97
94
1:16
Range (%)
99-115
97-113
83-98
Average (%)
107
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
96
EDTA plasma (n=5)
99-113
105
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.67
381.24
1992.21
183.21
395.08
2189.28
Standard deviation
17.49
30.65
148.22
20.72
34.93
143.84
C V (%)
9.37
8.04
7.44
11.31
8.84
6.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Fcγ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Fcγ in samples. No significant cross-reactivity or interference between Human Fcγ and analogues was observed.
