Human FcgammaBP (Fc Fragment of IgG Binding Protein) CLIA Kit
The Human FcGammaBP (FC Fragment of IgG Binding Protein) CLIA Kit is a cutting-edge assay designed for the accurate measurement of FcGammaBP levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications. FcGammaBP is a key protein involved in the binding and regulation of IgG antibodies, playing a crucial role in the immune response.
Dysregulation of FcGammaBP has been linked to various autoimmune diseases, cancer, and inflammatory disorders, making it a valuable biomarker for investigating these conditions and developing potential interventions. With its advanced technology and user-friendly design, the Human FcGammaBP CLIA Kit is an essential tool for researchers and healthcare professionals looking to explore the role of FcGammaBP in health and disease.
Product Name:
Human FcgammaBP (Fc Fragment of IgG Binding Protein) CLIA Kit
SKU:
HUES00273
Target:
Human FcgammaBP (Fc Fragment of IgG Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FcγBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FcγBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FcγBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FcγBP. You can calculate the concentration of Human FcγBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
95-108
99-112
Average (%)
108
100
106
1:4
Range (%)
84-96
100-116
99-116
Average (%)
91
106
106
1:8
Range (%)
101-120
99-117
99-117
Average (%)
109
107
107
1:16
Range (%)
87-102
98-116
86-101
Average (%)
93
106
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
90-105
98
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.83
410.52
1856.61
206.95
399.39
1890.87
Standard deviation
25.09
42.45
155.4
17.88
36.14
155.43
C V (%)
11.68
10.34
8.37
8.64
9.05
8.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FcγBP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FcγBP in samples. No significant cross-reactivity or interference between Human FcγBP and analogues was observed.
