The Human FCN1 (Ficolin-1) CLIA Kit is a highly reliable and sensitive assay designed for the precise measurement of FCN1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is essential for researchers studying the role of FCN1 in various biological processes.FCN1 is a key protein involved in the immune system, specifically in the activation of the lectin pathway of the complement system. It plays a crucial role in recognizing and binding to pathogens, promoting their clearance from the body.
Abnormal levels of FCN1 have been associated with various inflammatory and autoimmune diseases, highlighting its importance as a biomarker for diagnostic and therapeutic purposes.With its high sensitivity and specificity, the Human FCN1 CLIA Kit offers researchers a reliable tool for accurately measuring FCN1 levels in human samples, aiding in the advancement of scientific research and the development of new treatment strategies.
Product Name:
Human FCN1 (Ficolin 1) CLIA Kit
SKU:
HUES00272
Target:
Human FCN1 (Ficolin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FCN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FCN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FCN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FCN1. You can calculate the concentration of Human FCN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
90-102
103-118
Average (%)
105
97
109
1:4
Range (%)
94-105
88-100
102-118
Average (%)
99
95
109
1:8
Range (%)
97-110
97-115
87-100
Average (%)
104
105
93
1:16
Range (%)
93-106
99-113
92-107
Average (%)
100
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
98-111
104
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.89
248.25
947.31
94.87
250.57
976.47
Standard deviation
10.68
29.47
104.01
8.07
27.79
104.29
C V (%)
11.26
11.87
10.98
8.51
11.09
10.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FCN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FCN1 in samples. No significant cross-reactivity or interference between Human FCN1 and analogues was observed.
