Human FDP (Fibrinogen Degradation Product) ELISA Kit
The Human FDP (Fibrinogen Degradation Product) ELISA Kit is a powerful tool for accurately measuring levels of FDP in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Fibrinogen degradation products are important indicators of fibrinolysis and coagulation activity in the body. Elevated levels of FDP are associated with conditions such as disseminated intravascular coagulation (DIC), sepsis, and thrombosis, making this ELISA kit invaluable for the study of these diseases and the development of potential therapies.
Overall, the Human FDP ELISA Kit offers researchers a reliable and precise method for quantifying FDP levels, helping to advance our understanding of coagulation disorders and supporting the discovery of new treatment options.
Product Name:
Human FDP (Fibrinogen Degradation Product) ELISA Kit
SKU:
HUES03089
Target:
Human FDP (Fibrinogen Degradation Product)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FDP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FDP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FDP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FDP. You can calculate the concentration of Human FDP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
94-107
99-111
Average (%)
102
99
104
1:4
Range (%)
86-98
84-97
82-94
Average (%)
92
89
88
1:8
Range (%)
87-101
86-100
81-94
Average (%)
94
92
88
1:16
Range (%)
89-105
84-94
84-95
Average (%)
97
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.44
13.66
43.98
5.06
13.57
46.68
Standard deviation
0.35
0.67
2.32
0.3
0.68
1.88
C V (%)
6.43
4.9
5.28
5.93
5.01
4.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FDP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FDP in samples. No significant cross-reactivity or interference between Human FDP and analogues was observed.
