The Human Fetuin-A (Fetua) CLIA Kit is specially designed for the precise measurement of fetuin-A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it suitable for various research purposes.Fetuin-A, also known as alpha-2-HS-glycoprotein, is a key protein involved in regulating calcium metabolism and inhibiting ectopic calcification. It plays a crucial role in conditions such as diabetes, cardiovascular diseases, and chronic kidney disease, making it a valuable biomarker for investigating these conditions and developing potential treatments.
This CLIA kit provides researchers with a reliable tool for studying the role of fetuin-A in various physiological and pathological processes, offering valuable insights into its potential as a therapeutic target. Explore the functions of fetuin-A and its implications in disease pathology with the Human Fetuin-A CLIA Kit.
Product Name:
Human FETUA (Fetuin A) CLIA Kit
SKU:
HUES00276
Target:
Human FETUA (Fetuin A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FETUA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FETUA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FETUA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FETUA. You can calculate the concentration of Human FETUA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
92-103
84-96
Average (%)
93
97
90
1:4
Range (%)
96-111
88-100
88-100
Average (%)
102
93
93
1:8
Range (%)
95-108
89-101
100-113
Average (%)
102
96
107
1:16
Range (%)
101-115
86-100
100-118
Average (%)
108
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
107
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.37
16.07
42.83
5.13
17.63
41.57
Standard deviation
0.51
1.17
3.95
0.48
2.0
3.69
C V (%)
9.5
7.28
9.22
9.36
11.34
8.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FETUA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FETUA in samples. No significant cross-reactivity or interference between Human FETUA and analogues was observed.
