The Human FETUA (Fetuin-A) ELISA Kit is specifically designed for the precise measurement of fetuin-A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and trustworthy results for various research purposes.Fetuin-A is a key protein that plays a vital role in regulating calcium ion concentration and inhibiting calcification in the body. It is involved in various physiological processes, including bone metabolism, insulin sensitivity, and inflammation.
Abnormal fetuin-A levels have been linked to conditions such as diabetes, cardiovascular disease, and chronic kidney disease, making it a valuable biomarker for studying these disorders and exploring potential treatment options.Overall, the Human FETUA (Fetuin-A) ELISA Kit is a valuable tool for researchers looking to investigate the role of fetuin-A in health and disease, providing accurate and reliable data for groundbreaking discoveries in the field of biomedical research.
Product Name:
Human FETUA (Fetuin A) ELISA Kit
SKU:
HUES01601
Target:
Human FETUA (Fetuin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
5.63 ng/mL
Detection range:
9.38-600 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FETUA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FETUA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FETUA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FETUA. You can calculate the concentration of Human FETUA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
88-101
99-113
Average (%)
92
95
105
1:4
Range (%)
87-103
81-92
83-95
Average (%)
94
87
89
1:8
Range (%)
87-98
87-100
89-101
Average (%)
93
92
95
1:16
Range (%)
93-107
87-98
82-92
Average (%)
100
92
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
85-96
90
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
29.41
72.67
200.18
27.31
67.76
205.98
Standard deviation
1.96
3.68
8.49
1.6
3.75
7.29
C V (%)
6.66
5.06
4.24
5.86
5.53
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FETUA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FETUA in samples. No significant cross-reactivity or interference between Human FETUA and analogues was observed.
