The Human Fetuin-B (FetuB) ELISA Kit is specifically engineered for the precise measurement of Fetuin-B levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.Fetuin-B, also known as FetuB, is a vital protein associated with various physiological processes, including lipid metabolism, insulin resistance, and inflammatory responses.
Its dysregulation has been linked to several diseases, such as diabetes, obesity, and cardiovascular disorders, highlighting its significance as a biomarker for disease progression and potential therapeutic interventions.By utilizing the Human Fetuin-B ELISA Kit, researchers can effectively investigate the role of Fetuin-B in health and disease, paving the way for innovative discoveries and advancements in the field of biomedical research.
Product Name:
Human Fetuin B (FETUB) ELISA Kit
Product Code:
HUDL01046
Size:
96 Assays
Target:
FETUB
Synonyms:
FETU-B, 16G2, Gugu, IRL685, Fetuin-like protein IRL685
Detection Method:
Sandwich
Range:
93.75-6000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
