Human FGF18 (Fibroblast Growth Factor 18) ELISA Kit
The Human FGF18 (Fibroblast Growth Factor 18) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of FGF18 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.FGF18 is a key regulator of cell growth, differentiation, and tissue repair, playing a crucial role in various physiological processes.
It is implicated in conditions such as skeletal development, wound healing, and organogenesis, making it a valuable biomarker for studying these processes and potential therapeutic interventions.Overall, the Human FGF18 ELISA Kit is a valuable tool for researchers looking to study the role of FGF18 in health and disease and develop novel treatment strategies.
Product Name:
Human FGF18 (Fibroblast Growth Factor 18) ELISA Kit
SKU:
HUES03435
Target:
Human FGF18 (Fibroblast Growth Factor 18)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF18. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF18, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF18. You can calculate the concentration of Human FGF18 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
91-106
91-108
Average (%)
90
97
99
1:4
Range (%)
89-103
82-95
87-102
Average (%)
96
89
94
1:8
Range (%)
92-106
82-94
87-100
Average (%)
97
88
93
1:16
Range (%)
91-104
81-93
86-100
Average (%)
97
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
91
EDTA plasma (n=5)
97-107
102
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.84
110.71
346.06
49.18
102.01
354.77
Standard deviation
2.98
5.17
18.55
3.14
5.8
17.31
C V (%)
6.5
4.67
5.36
6.38
5.69
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF18 in samples. No significant cross-reactivity or interference between Human FGF18 and analogues was observed.
