Human FGF19 (Fibroblast Growth Factor 19) CLIA Kit
The Human FGF19 (Fibroblast Growth Factor 19) CLIA Kit is a highly reliable assay designed for the precise measurement of FGF19 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FGF19 is a critical growth factor that plays a key role in regulating metabolic processes, including bile acid synthesis and glucose homeostasis.
Dysregulation of FGF19 levels has been linked to various metabolic disorders, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and proven performance, the Human FGF19 CLIA Kit from Assay Genie is a valuable tool for researchers looking to explore the role of FGF19 in health and disease. Trust in the reliability and precision of this kit to drive your research forward.
Product Name:
Human FGF19 (Fibroblast Growth Factor 19) CLIA Kit
SKU:
HUES00071
Target:
Human FGF19 (Fibroblast Growth Factor 19)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF19. You can calculate the concentration of Human FGF19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
102-113
98-112
Average (%)
100
108
105
1:4
Range (%)
101-114
93-106
92-103
Average (%)
108
99
97
1:8
Range (%)
89-102
92-107
96-111
Average (%)
96
99
104
1:16
Range (%)
102-118
90-101
87-99
Average (%)
110
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
89-106
97
Cell culture media (n=5)
92-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.89
58.71
175.5
24.55
54.02
169.53
Standard deviation
2.78
5.47
13.07
2.14
5.89
19.09
C V (%)
11.64
9.32
7.45
8.72
10.9
11.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF19 in samples. No significant cross-reactivity or interference between Human FGF19 and analogues was observed.
