The Human FGF4 (Fibroblast Growth Factor 4) CLIA Kit is specifically designed for the accurate measurement of FGF4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.FGF4 is a key growth factor that plays a critical role in cell proliferation, differentiation, and tissue development. It is also involved in various physiological processes such as wound healing, embryonic development, and tissue repair.
Dysregulation of FGF4 has been associated with conditions like cancer, developmental disorders, and metabolic diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human FGF4 CLIA Kit from Assay Genie provides researchers with a powerful tool for studying FGF4 biology and its role in health and disease.
Product Name:
Human FGF4 (Fibroblast Growth Factor 4) CLIA Kit
SKU:
HUES00380
Target:
Human FGF4 (Fibroblast Growth Factor 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF4. You can calculate the concentration of Human FGF4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
88-99
89-101
Average (%)
101
94
96
1:4
Range (%)
97-113
101-114
88-101
Average (%)
103
107
93
1:8
Range (%)
85-99
86-100
85-97
Average (%)
92
92
92
1:16
Range (%)
89-104
101-116
97-113
Average (%)
96
108
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
90-106
97
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.21
92.16
471.86
50.19
98.25
495.9
Standard deviation
6.24
9.43
36.05
4.2
11.55
54.35
C V (%)
12.19
10.23
7.64
8.37
11.76
10.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF4 in samples. No significant cross-reactivity or interference between Human FGF4 and analogues was observed.
