The Human FGF4 (Fibroblast Growth Factor 4) ELISA Kit is designed to quantitatively measure the levels of FGF4 in various human biological samples. Fibroblast Growth Factor 4, known as FGF4, is a critical signaling protein that plays a crucial role in cell growth, differentiation, and development. It is involved in processes such as embryogenesis, wound healing, and tissue repair. Accurate measurement of FGF4 levels is essential for understanding its role in cellular signaling pathways and its impact on growth regulation.
This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and is user-friendly, making it suitable for both research and clinical applications. Rely on Assay Genie's FGF4 ELISA Kit for dependable quantification of this important growth factor in your studies.
Product Name:
Human FGF4 (Fibroblast Growth Factor 4) ELISA Kit
SKU:
AEES00079
Target:
Human FGF4 (Fibroblast Growth Factor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF4. You can calculate the concentration of Human FGF4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
91-104
99-107
Average (%)
104
97
102
1:4
Range (%)
95-106
82-96
92-102
Average (%)
100
90
99
1:8
Range (%)
91-99
98-108
87-101
Average (%)
94
101
94
1:16
Range (%)
92-100
85-98
89-102
Average (%)
96
91
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-98
94
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
95-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.39
107.11
479.28
45.2
115.26
487.2
Standard deviation
2.48
5.01
21.09
2.4
5.76
24.02
C V (%)
5.99
4.68
4.4
5.31
5.0
4.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF4 in samples. No significant cross-reactivity or interference between Human FGF4 and analogues was observed.
