The Human FGF6 (Fibroblast Growth Factor 6) CLIA Kit is a cutting-edge assay designed for the accurate measurement of FGF6 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.FGF6 is a key growth factor involved in cell proliferation, tissue repair, and development. It plays a crucial role in various physiological processes, making it a valuable biomarker for studying conditions such as wound healing, tissue regeneration, and cancer.
By accurately quantifying FGF6 levels, researchers can gain insights into disease mechanisms and potential therapeutic interventions.The Human FGF6 CLIA Kit from AssayGenie provides researchers with a reliable tool for studying the role of FGF6 in health and disease. With its high performance and ease of use, this kit is a valuable resource for advancing our understanding of FGF6 biology and developing novel treatments for FGF6-related conditions.
Product Name:
Human FGF6 (Fibroblast Growth Factor 6) CLIA Kit
SKU:
HUES00381
Target:
Human FGF6 (Fibroblast Growth Factor 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGF6. You can calculate the concentration of Human FGF6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
94-107
85-96
Average (%)
99
101
91
1:4
Range (%)
88-102
99-115
100-114
Average (%)
95
107
106
1:8
Range (%)
98-113
97-113
84-99
Average (%)
106
105
91
1:16
Range (%)
92-104
88-105
89-104
Average (%)
98
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
95-111
102
Cell culture media (n=5)
101-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.84
241.06
994.17
99.62
238.65
901.77
Standard deviation
9.93
18.1
102.8
10.95
16.87
92.34
C V (%)
9.66
7.51
10.34
10.99
7.07
10.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGF6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF6 in samples. No significant cross-reactivity or interference between Human FGF6 and analogues was observed.
