The Human FGF7 (Fibroblast Growth Factor 7) ELISA Kit is a powerful tool for detecting and quantifying FGF7 levels in human biological samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides accurate and precise results, making it perfect for a wide range of research applications.FGF7 is a key growth factor that plays a critical role in cell proliferation, tissue repair, and wound healing.
Its dysregulation has been linked to various diseases, including cancer and skin disorders, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.By utilizing the Human FGF7 ELISA Kit, researchers can gain valuable insights into the role of FGF7 in disease pathogenesis and explore potential treatment strategies. Trust in this kit to deliver reliable and reproducible data for your research needs.
Product Name:
Human FGF7 (Fibroblast Growth Factor 7) ELISA Kit
SKU:
HUES01378
Target:
Human FGF7 (Fibroblast Growth Factor 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FGF7/KGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF7/KGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF7/KGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FGF7/KGF. You can calculate the concentration of Human FGF7/KGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
89-103
86-100
Average (%)
95
95
93
1:4
Range (%)
88-100
85-98
90-103
Average (%)
95
92
95
1:8
Range (%)
90-105
81-94
81-93
Average (%)
98
86
88
1:16
Range (%)
92-108
86-99
87-98
Average (%)
99
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.69
217.61
889.61
96.83
210.35
850.06
Standard deviation
6.19
9.29
39.77
6.35
11.51
25.59
C V (%)
6.15
4.27
4.47
6.56
5.47
3.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FGF7/KGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGF7/KGF in samples. No significant cross-reactivity or interference between Human FGF7/KGF and analogues was observed.
