Human FGFR1 (Fibroblast Growth Factor Receptor 1) CLIA Kit
The Human FGFR1 (Fibroblast Growth Factor Receptor 1) CLIA Kit is specifically designed for the precise quantification of FGFR1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.FGFR1 is a critical receptor involved in various cellular processes, including cell growth, differentiation, and wound healing. Dysregulation of FGFR1 signaling has been implicated in numerous diseases, such as cancer, developmental disorders, and skeletal abnormalities.
Understanding the role of FGFR1 in these conditions can provide valuable insights for therapeutic interventions.With its reliable performance and user-friendly format, the Human FGFR1 CLIA Kit from Assay Genie is an indispensable tool for researchers studying FGFR1-related pathways and diseases. Trust in this kit to deliver precise and reproducible data for advancing your research endeavors.
Product Name:
Human FGFR1 (Fibroblast Growth Factor Receptor 1) CLIA Kit
SKU:
HUES00245
Target:
Human FGFR1 (Fibroblast Growth Factor Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGFR1. You can calculate the concentration of Human FGFR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
91-108
90-102
Average (%)
102
98
96
1:4
Range (%)
89-102
100-116
84-96
Average (%)
95
108
90
1:8
Range (%)
101-117
103-117
99-112
Average (%)
108
109
105
1:16
Range (%)
97-109
85-99
100-114
Average (%)
103
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
89-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.08
626.91
1688.77
205.04
624.69
1754.11
Standard deviation
21.29
55.04
135.61
17.55
50.16
188.04
C V (%)
9.72
8.78
8.03
8.56
8.03
10.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGFR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR1 in samples. No significant cross-reactivity or interference between Human FGFR1 and analogues was observed.
