Human FGFR2 (Fibroblast Growth Factor Receptor 2) CLIA Kit
The Human FGFR2 (Fibroblast Growth Factor Receptor 2) CLIA Kit is specifically designed for the precise measurement of FGFR2 levels in human samples such as serum, plasma, and tissue extracts. This kit offers exceptional sensitivity and accuracy, ensuring consistent and trustworthy results for various research purposes.FGFR2 is a key receptor involved in cell growth, differentiation, and tissue repair processes. Dysregulation of FGFR2 signaling has been linked to numerous diseases, including cancer, skeletal disorders, and developmental abnormalities.
By detecting and quantifying FGFR2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human FGFR2 CLIA Kit from Assay Genie provides a reliable tool for studying FGFR2 biology and its implications in health and disease.
Product Name:
Human FGFR2 (Fibroblast Growth Factor Receptor 2) CLIA Kit
SKU:
HUES00138
Target:
Human FGFR2 (Fibroblast Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGFR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGFR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGFR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FGFR2. You can calculate the concentration of Human FGFR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
94-109
101-120
Average (%)
108
101
109
1:4
Range (%)
98-116
89-99
93-108
Average (%)
106
94
99
1:8
Range (%)
97-109
89-103
89-103
Average (%)
103
96
95
1:16
Range (%)
95-109
92-103
97-113
Average (%)
101
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.85
96.8
304.95
42.49
101.07
276.84
Standard deviation
3.66
7.18
19.79
4.97
8.48
18.66
C V (%)
8.54
7.42
6.49
11.7
8.39
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FGFR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FGFR2 in samples. No significant cross-reactivity or interference between Human FGFR2 and analogues was observed.
