Human Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit
The Human Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit is specifically designed for the precise measurement of FGFR4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for a variety of research applications.FGFR4 is a key receptor involved in cell growth, differentiation, and survival, playing a crucial role in various biological processes. Dysregulation of FGFR4 has been linked to numerous diseases, including cancer, metabolic disorders, and developmental abnormalities, highlighting its significance as a potential therapeutic target and biomarker.
Suitable for use in academic research, drug discovery, and clinical diagnostics, the Human FGFR4 ELISA Kit provides researchers with a reliable and efficient method for studying the role of FGFR4 in health and disease. Order now to unlock new insights into the biology of FGFR4 and pave the way for innovative treatments and interventions.
Product Name:
Human Fibroblast Growth Factor Receptor 4 (FGFR4) ELISA Kit
Product Code:
HUDL01071
Size:
96 Assays
Target:
FGFR4
Synonyms:
CD334, JTK2, TKF
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
