The Human FLN Beta (Filamin B Beta) CLIA Kit is specifically designed for the precise measurement of Filamin B Beta levels in human biological samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers exceptional accuracy, sensitivity, and specificity, ensuring consistent and dependable results for a variety of research applications.Filamin B Beta is a key cytoskeletal protein that plays a critical role in cell adhesion, migration, and signaling pathways. Dysregulation of Filamin B Beta has been implicated in various diseases, including cancer, developmental disorders, and cardiovascular conditions.
As such, monitoring Filamin B Beta levels can provide valuable insights into disease progression and potential therapeutic targets.Overall, the Human FLN Beta (Filamin B Beta) CLIA Kit is a valuable tool for researchers seeking to investigate the role of Filamin B Beta in health and disease, ultimately contributing to the advancement of medical knowledge and the development of novel treatment strategies.
Product Name:
Human FLN beta (Filamin B, Beta) CLIA Kit
SKU:
HUES01117
Target:
Human FLN beta (Filamin B, Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FLNβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FLNβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FLNβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FLNβ. You can calculate the concentration of Human FLNβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
92-104
90-105
Average (%)
95
97
96
1:4
Range (%)
96-110
85-98
98-112
Average (%)
103
91
106
1:8
Range (%)
95-112
93-106
93-106
Average (%)
103
99
98
1:16
Range (%)
92-104
97-110
91-104
Average (%)
97
103
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-104
99
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
99-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.46
549.44
1535.63
221.24
523.93
1556.8
Standard deviation
18.17
60.49
175.06
21.55
39.4
153.97
C V (%)
8.28
11.01
11.4
9.74
7.52
9.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FLNβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FLNβ in samples. No significant cross-reactivity or interference between Human FLNβ and analogues was observed.
