The Human FLNC (Filamin C Gamma) ELISA Kit from AssayGenie is a cutting-edge tool designed for the accurate measurement of FLNC levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable asset for a wide range of research applications.Filamin C Gamma, also known as FLNC, is a vital protein involved in various cellular functions, including cell structure and signaling pathways.
Dysregulation of FLNC has been linked to numerous diseases such as muscle disorders, cardiovascular diseases, and neurological conditions, highlighting its significance as a biomarker for disease diagnosis and therapy development.Trust AssayGenie's Human FLNC ELISA Kit to provide you with the precise and accurate data you need for your research endeavors. Order yours today and unlock new insights into the role of Filamin C Gamma in human health and disease.
Product Name:
Human FLNC (Filamin C, Gamma) ELISA Kit
SKU:
HUES03006
Target:
Human FLNC (Filamin C, Gamma)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FLNC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FLNC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FLNC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FLNC. You can calculate the concentration of Human FLNC in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
95-111
99-111
Average (%)
95
103
104
1:4
Range (%)
90-104
87-98
84-94
Average (%)
96
93
89
1:8
Range (%)
86-98
83-94
84-94
Average (%)
93
88
89
1:16
Range (%)
89-104
83-97
85-99
Average (%)
95
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
95
EDTA plasma (n=5)
85-99
90
Cell culture media (n=5)
91-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
3.21
9.35
1.17
3.28
9.18
Standard deviation
0.06
0.16
0.46
0.06
0.19
0.45
C V (%)
5.56
4.98
4.92
5.13
5.79
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FLNC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FLNC in samples. No significant cross-reactivity or interference between Human FLNC and analogues was observed.
