The Human Flot2 (Flotillin-2) CLIA Kit is specifically designed to quantitatively measure Flotillin-2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and reproducible results for a variety of research applications.Flotillin-2 is a protein that plays a critical role in cell membrane dynamics and signal transduction. It is involved in various cellular processes such as endocytosis, cell adhesion, and intracellular trafficking. Dysregulation of Flotillin-2 expression has been linked to various diseases including cancer, Alzheimer's disease, and cardiovascular disorders, making it a valuable biomarker for disease research and potential therapeutic development.
By using the Human Flot2 CLIA Kit, researchers can gain insight into the role of Flotillin-2 in disease pathology and potentially identify new therapeutic targets. The high sensitivity and specificity of this kit make it an essential tool for studying Flotillin-2 biology and its implications in human health.
Product Name:
Human FLOT2 (Flotillin 2) CLIA Kit
SKU:
HUES00278
Target:
Human FLOT2 (Flotillin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FLOT2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FLOT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FLOT2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FLOT2. You can calculate the concentration of Human FLOT2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
85-99
98-113
Average (%)
100
91
106
1:4
Range (%)
98-116
97-114
103-120
Average (%)
106
104
110
1:8
Range (%)
86-97
101-115
92-104
Average (%)
91
109
97
1:16
Range (%)
98-110
103-118
95-107
Average (%)
103
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
103
EDTA plasma (n=5)
96-110
104
Cell culture media (n=5)
100-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
0.87
4.26
0.45
0.84
3.95
Standard deviation
0.04
0.1
0.39
0.04
0.09
0.38
C V (%)
8.16
11.49
9.15
8.89
10.71
9.62
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FLOT2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human FLOT2 in samples. No significant cross-reactivity or interference between Human FLOT2 and analogues was observed.
