Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) CLIA Kit
The Human FLT3L (FMS-like Tyrosine Kinase 3 Ligand) CLIA Kit is specifically designed for the accurate detection of FLT3L levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.FLT3L is a key growth factor that plays a crucial role in the development and differentiation of immune cells, particularly dendritic cells.
Its dysregulation has been implicated in various autoimmune diseases, leukemias, and other disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and user-friendly design, the Human FLT3L CLIA Kit from Assay Genie is a valuable tool for researchers and clinicians alike, offering accurate and actionable insights into FLT3L levels in human samples.
Product Name:
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) CLIA Kit
SKU:
HUES00073
Target:
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Flt3L. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Flt3L and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Flt3L, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Flt3L. You can calculate the concentration of Human Flt3L in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
95-110
83-98
Average (%)
94
101
90
1:4
Range (%)
93-106
94-107
84-96
Average (%)
100
102
91
1:8
Range (%)
99-115
89-104
91-106
Average (%)
107
97
98
1:16
Range (%)
97-115
96-114
99-116
Average (%)
105
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
97-110
105
Cell culture media (n=5)
99-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.22
42.6
226.45
26.78
46.32
221.91
Standard deviation
2.21
4.17
25.29
3.21
5.49
13.36
C V (%)
8.12
9.79
11.17
11.99
11.85
6.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Flt3L concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed.
