The Human FOXC1 (Forkhead Box Protein C1) CLIA Kit is specifically designed for the accurate detection of FOXC1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.FOXC1 is a critical transcription factor that plays a role in various biological processes, including development, cell proliferation, and differentiation. Dysregulation of FOXC1 has been associated with a range of diseases and conditions, such as cancer, glaucoma, and congenital heart defects, making it a valuable biomarker for studying these ailments and potential therapeutic interventions.
Overall, the Human FOXC1 CLIA Kit is a powerful tool for researchers looking to investigate the role of FOXC1 in different biological processes and diseases, providing valuable insights into its functions and potential clinical applications.
Product Name:
Human FOXC1 (Forkhead Box Protein C1) CLIA Kit
SKU:
HUES00646
Target:
Human FOXC1 (Forkhead Box Protein C1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FOXC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FOXC1. You can calculate the concentration of Human FOXC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
96-110
88-103
Average (%)
100
103
94
1:4
Range (%)
103-118
95-108
87-101
Average (%)
109
103
94
1:8
Range (%)
86-99
88-102
99-112
Average (%)
92
95
105
1:16
Range (%)
99-117
91-106
92-106
Average (%)
107
99
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
99
EDTA plasma (n=5)
99-114
104
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.56
289.44
701.87
102.8
277.31
748.52
Standard deviation
10.2
23.07
59.94
11.07
32.45
62.88
C V (%)
9.48
7.97
8.54
10.77
11.7
8.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FOXC1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FOXC1 in samples. No significant cross-reactivity or interference between Human FOXC1 and analogues was observed.
