The Human FOXO1 (Forkhead Box Protein O1) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of FOXO1 levels in human samples, including serum, plasma, and cell culture supernatants. With its reliable and reproducible results, this kit is perfect for various research applications.FOXO1 is a key transcription factor that regulates various cellular processes, including cell growth, differentiation, and metabolic pathways.
Dysregulation of FOXO1 has been linked to various diseases, including cancer, diabetes, and cardiovascular disorders. Therefore, measuring FOXO1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human FOXO1 CLIA Kit offers researchers a powerful tool for studying FOXO1-related pathways and diseases, providing accurate and quantitative measurements for in-depth analysis.
Product Name:
Human FOXO1 (Forkhead Box Protein O1) CLIA Kit
SKU:
HUES00647
Target:
Human FOXO1 (Forkhead Box Protein O1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FOXO1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXO1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FOXO1. You can calculate the concentration of Human FOXO1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
95-107
89-100
Average (%)
93
101
95
1:4
Range (%)
84-97
89-103
100-117
Average (%)
91
96
107
1:8
Range (%)
95-109
97-112
95-111
Average (%)
102
104
101
1:16
Range (%)
91-106
85-99
96-113
Average (%)
97
91
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
91-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.21
260.74
863.09
102.98
277.95
911.42
Standard deviation
9.97
23.21
68.44
9.28
32.24
81.66
C V (%)
9.05
8.9
7.93
9.01
11.6
8.96
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FOXO1 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human FOXO1 in samples. No significant cross-reactivity or interference between Human FOXO1 and analogues was observed.
