The Human FOXO3 (Forkhead Box Protein O3) CLIA Kit is a reliable and accurate tool for the detection of FOXO3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.FOXO3 is a critical transcription factor involved in regulating cell cycle progression, oxidative stress response, and longevity. It plays a key role in various biological processes including apoptosis, DNA repair, and metabolism.
Dysregulation of FOXO3 has been linked to aging, cancer, and age-related diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human FOXO3 CLIA Kit provides researchers with a valuable tool for studying the role of FOXO3 in human health and disease, offering reliable results that can contribute to a deeper understanding of FOXO3 biology and its potential implications for clinical applications.
Product Name:
Human FOXO3 (Forkhead Box Protein O3) CLIA Kit
SKU:
HUES00648
Target:
Human FOXO3 (Forkhead Box Protein O3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FOXO3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXO3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXO3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FOXO3. You can calculate the concentration of Human FOXO3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
87-100
90-105
Average (%)
101
93
97
1:4
Range (%)
92-104
90-102
83-98
Average (%)
99
97
90
1:8
Range (%)
89-102
98-110
88-100
Average (%)
95
105
93
1:16
Range (%)
92-109
87-102
90-105
Average (%)
100
94
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-116
108
EDTA plasma (n=5)
96-106
101
Cell culture media (n=5)
97-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.94
444.82
1789.37
214.64
436.74
1926.62
Standard deviation
24.49
48.35
184.48
21.36
50.53
136.21
C V (%)
11.61
10.87
10.31
9.95
11.57
7.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FOXO3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FOXO3 in samples. No significant cross-reactivity or interference between Human FOXO3 and analogues was observed.
