The Human FPA (Fibrinopeptide A) CLIA Kit is specifically designed for the precise measurement of FPA levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results for a variety of research purposes.Fibrinopeptide A is a key component in the blood clotting process, playing a crucial role in fibrin formation and clot stabilization. Abnormal levels of FPA have been associated with various medical conditions such as thrombosis, cardiovascular diseases, and inflammatory disorders.
Therefore, this kit is essential for studying the pathophysiology of these conditions and evaluating potential therapeutic interventions.Overall, the Human FPA CLIA Kit from Assay Genie is a valuable tool for researchers and clinicians seeking to investigate the role of Fibrinopeptide A in health and disease. Unleash the potential of your research with this cutting-edge assay kit.
Product Name:
Human FPA (Fibrinopeptide A) CLIA Kit
SKU:
HUES01157
Target:
Human FPA (Fibrinopeptide A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FPA. You can calculate the concentration of Human FPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
88-104
91-104
Average (%)
104
96
98
1:4
Range (%)
87-99
103-115
84-95
Average (%)
93
109
90
1:8
Range (%)
96-113
91-107
91-102
Average (%)
104
98
96
1:16
Range (%)
89-106
86-99
102-114
Average (%)
97
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
98-114
104
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.92
3.05
9.11
0.92
2.93
9.05
Standard deviation
0.08
0.3
1.03
0.08
0.27
0.56
C V (%)
8.7
9.84
11.31
8.7
9.22
6.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FPA in samples. No significant cross-reactivity or interference between Human FPA and analogues was observed.
