The Human FPR2 (Formyl Peptide Receptor 2) CLIA Kit is designed for the precise measurement of FPR2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research purposes.FPR2 is a key receptor involved in immune response and inflammation, playing a critical role in modulating various cellular processes.
Dysregulation of FPR2 has been implicated in multiple diseases, including inflammatory disorders, infections, and cancer, highlighting its significance as a potential biomarker for disease research and therapeutic development.With its reliable performance and broad application range, the Human FPR2 CLIA Kit is a valuable tool for scientists and researchers investigating the role of FPR2 in health and disease.
Product Name:
Human FPR2 (Formyl Peptide Receptor 2) CLIA Kit
SKU:
HUES00840
Target:
Human FPR2 (Formyl Peptide Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FPR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FPR2. You can calculate the concentration of Human FPR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
93-108
101-113
Average (%)
90
100
107
1:4
Range (%)
91-105
97-114
102-116
Average (%)
97
105
108
1:8
Range (%)
100-111
93-106
88-101
Average (%)
106
99
94
1:16
Range (%)
88-104
87-98
102-114
Average (%)
95
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
99-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.07
114.53
429.7
47.94
104.56
397.6
Standard deviation
4.52
11.11
44.86
4.52
7.47
28.43
C V (%)
9.6
9.7
10.44
9.43
7.14
7.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FPR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FPR2 in samples. No significant cross-reactivity or interference between Human FPR2 and analogues was observed.
