Human FSH (Follicle-Stimulating Hormone) ELISA Kit
The Human FSH (Follicle Stimulating Hormone) ELISA Kit is a specialized assay designed for the accurate measurement of FSH levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it well-suited for a variety of research applications.FSH is a key hormone involved in the regulation of reproductive processes, specifically in the development and maturation of ovarian follicles in women and sperm production in men.
Abnormal FSH levels can indicate issues with fertility, hormonal imbalances, or other reproductive disorders, making it an important biomarker for studying these conditions and developing targeted treatment strategies.By utilizing the Human FSH ELISA Kit, researchers can accurately measure FSH levels in samples, leading to a better understanding of reproductive health and potential therapies for related conditions. This kit provides a reliable and efficient tool for studying FSH levels in various research settings.
Product Name:
Human FSH (Follicle-Stimulating Hormone) ELISA Kit
SKU:
HUES02229
Target:
Human FSH (Follicle-Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FSH. You can calculate the concentration of Human FSH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
96-112
90-104
Average (%)
96
103
96
1:4
Range (%)
100-112
85-96
91-104
Average (%)
106
90
99
1:8
Range (%)
99-115
82-95
93-108
Average (%)
107
89
100
1:16
Range (%)
95-107
83-95
91-104
Average (%)
101
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
90-101
95
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.9
12.2
42.6
4.9
11.1
46.2
Standard deviation
0.3
0.6
1.6
0.3
0.6
1.5
C V (%)
6.12
4.92
3.76
6.12
5.41
3.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSH in samples. No significant cross-reactivity or interference between Human FSH and analogues was observed.
