Human FSHR (Follicle Stimulating Hormone Receptor) CLIA Kit
The Human FSHR (Follicle-Stimulating Hormone Receptor) CLIA Kit is a powerful tool for the precise quantification of FSHR levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors.FSHR is a key receptor involved in follicle-stimulating hormone signaling, playing a critical role in reproductive functions such as follicle development and ovulation.
Dysregulation of FSHR activity is associated with various reproductive disorders, making it a crucial target for study and potential therapeutic interventions.With its superior performance and broad applicability, the Human FSHR CLIA Kit is an indispensable tool for investigating FSHR-related mechanisms and exploring new avenues for reproductive health research. Trust in its precision and reliability to propel your research forward.
Product Name:
Human FSHR (Follicle Stimulating Hormone Receptor) CLIA Kit
SKU:
HUES00658
Target:
Human FSHR (Follicle Stimulating Hormone Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FSHR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSHR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSHR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FSHR. You can calculate the concentration of Human FSHR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
93-106
92-102
Average (%)
104
99
97
1:4
Range (%)
101-116
90-104
103-121
Average (%)
109
96
110
1:8
Range (%)
89-102
89-102
89-103
Average (%)
94
95
94
1:16
Range (%)
94-106
92-107
95-108
Average (%)
100
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
99-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.38
597.39
1433.45
206.56
551.54
1345.51
Standard deviation
21.27
57.11
93.03
19.29
54.22
140.34
C V (%)
10.83
9.56
6.49
9.34
9.83
10.43
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FSHR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSHR in samples. No significant cross-reactivity or interference between Human FSHR and analogues was observed.
