The Human FST (Follistatin) CLIA Kit is a powerful tool for researchers looking to accurately measure Follistatin levels in human samples. This kit is designed for use with serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for reliable and reproducible results.Follistatin is known for its role in regulating the activity of various growth factors, particularly activin and myostatin, making it a key player in various biological processes, including muscle development, inflammation, and tissue repair.
Abnormal Follistatin levels have been linked to conditions such as muscular dystrophy, infertility, and cancer, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With the Human FST CLIA Kit, researchers can confidently measure Follistatin levels in their samples, advancing their understanding of the role this protein plays in health and disease.
Product Name:
Human FST (Follistatin) CLIA Kit
SKU:
HUES00074
Target:
Human FST (Follistatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FST. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FST, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FST. You can calculate the concentration of Human FST in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
102-117
92-104
Average (%)
108
108
97
1:4
Range (%)
99-111
96-114
90-105
Average (%)
105
104
96
1:8
Range (%)
90-101
86-98
92-103
Average (%)
96
92
97
1:16
Range (%)
97-113
87-100
98-110
Average (%)
104
94
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
96-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.53
628.85
1873.24
202.18
650.75
1688.36
Standard deviation
17.91
63.7
178.52
21.19
54.53
181.84
C V (%)
9.02
10.13
9.53
10.48
8.38
10.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FST in samples. No significant cross-reactivity or interference between Human FST and analogues was observed.
