The Human FSTL1 (Follistatin-like protein 1) CLIA Kit is designed for the precise measurement of FSTL1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.FSTL1 is a key protein implicated in various cellular processes, including inflammation, tissue repair, and cartilage formation.
Dysregulation of FSTL1 has been associated with conditions such as arthritis, cardiovascular diseases, and fibrosis, highlighting its importance as a biomarker for studying these diseases and developing potential therapeutic interventions.With its reliable performance and versatility, the Human FSTL1 CLIA Kit is a valuable tool for researchers investigating the role of FSTL1 in health and disease.
Product Name:
Human FSTL1 (Follistatin Like Protein 1) CLIA Kit
SKU:
HUES00148
Target:
Human FSTL1 (Follistatin Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FSTL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FSTL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FSTL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FSTL1. You can calculate the concentration of Human FSTL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
96-113
97-111
Average (%)
102
103
103
1:4
Range (%)
94-107
94-108
93-105
Average (%)
100
102
99
1:8
Range (%)
98-110
92-104
89-101
Average (%)
103
97
95
1:16
Range (%)
100-113
89-100
102-118
Average (%)
106
94
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
98-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.77
413.14
1840.17
200.92
448.04
1660.55
Standard deviation
21.3
39.62
198.19
26.06
32.84
169.38
C V (%)
11.53
9.59
10.77
12.97
7.33
10.2
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FSTL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FSTL1 in samples. No significant cross-reactivity or interference between Human FSTL1 and analogues was observed.
